Genome, transcriptome and proteome analyses require pure samples to obtain reliable results. However, tissue preparations are usually inhomogeneous and consist of a mixture of different cells. As tissue complexity can affect the outcome and interpretation of molecular studies, isolation of pure cell populations is preferred. Using a laser coupled to a research microscope, defined target cells or even subcellular components like organelles and chromosomes, can be isolated. In our lab, a P.A.L.M. microbeam from Zeiss, an inverted microscope with a pulsed UVa-laser, is present. 

 

Advantages of laser capture microdissection

  • Obtaining pure cell populations without the risk of contamination
  • Genome, transcriptome and proteome analysis after isolation
  • Can be performed on different sample types such as snap frozen or formalin fixed paraffin-embedded tissue sections, cytospins, cell smears and chromosome preparations. 
  • Can be combined with fluorescence staining to visualize particular cells or cell organelles of interest

 

Workflow

You provide us a glass slide with the cells of interest, stained if necessary to visualize the appropriate cell types. These cells will be isolated directly in a DNA or RNA extraction buffer, upon which DNA or RNA analysis can be performed.

 

About ProGenTomics

ProGenTomics, located in Gent (Belgium), offers a wide range of proteomics and genomics services and support to the academic community and its external customers. We aim to tailor our services to the needs of our collaborators and customers, while giving great support.  

Contact us

ProGenTomics, proteomics & genomics services

  Ghent University, Faculty of Pharmaceutical Sciences

  Ottergemsesteenweg 460, 9000 Gent, Belgium

 

  +32 9 264 83 56