qPCR and RT-qPCR equipment

Precise real-time detection of PCR products by a fluorescent reporter molecule

Roche LightCycler480 I and II

All ProGenTomics (RT-)qPCR experiments are performed on a LightCycler480 I or II (Roche). This device allows a rapid and reliable analysis, both in a 96-well or 384-well format, or even in one or multiple 8-tube strips, thus enabling performing low throughput assays as well as high throughput experiments. Several detection methodologies are available, with their own assets and disadvantages. Non-specific general DNA-binding dyes such as SYBR Green or High Resolution Melting dyes on one hand, allow the generation of melting curves, offering an extra quality control of your results. Targeted fluorescent probes such as TaqMan assay on the other hand, yield the specific detection of the amplified product and make multiplexing possible.

State-of-the-art sample preparation for next-generation sequencing

State-of-the-art sample preparation equipment allowing for a lot of different sample preparations in an automated, efficient and accurate way.

Diagenode IP-Star

The Diagenode IP-Star is a compact automated system that provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. It allows for high-throughput sample preparation compatible with popular downstream applications such as qPCR and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purifications

Covaris S2

The Covaris S2 focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing for numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. The computer controlled device allows for shearing at a certain, set length.

Sequencing platforms

A wide range of sequencing apparatus allowing to sequence at the scale and scope of your project

MiSeq

The in-house MiSeq benchtop sequencer is well suited for smaller projects and thus allows for more focused applications such as targeted gene sequencing, metagenomics, small genome and transcriptome sequencing, and targeted gene expression. Generating 1 million up to 25 million reads, the MiSeq sequencer is ideal for reduced representation studies and narrowly focused applications.
More MiSeq specifications

NextSeq 500

With an output of up to 400 million reads, our in-house Illumina NextSeq 500 sequencer is more suited for larger study designs. It is the only benchtop sequencer that can sequence a 30x coverage human genome in one run. The NextSeq 500 is easily configured providing researchers perfect scalability for mid-sized to large projects at maximal operational efficiency.
More NextSeq500 specifications.

HiSeq 3000

The HiSeq 3000 System is a powerful and efficient ultra-high-throughput sequencing system that, with an output of up to 750 gigabase (Gb) of data, is ideally suited for large-scale genomics projects.
More HiSeq 3000 specifications.

PacBio RS

The PacBio RS is a Single Molecule, Real-Time (SMRT) DNA Sequencing System that provides the highest consensus accuracy and longest read lengths of, on average, up to 10,000 bp which makes it extermely suitable for whole-genome sequencing, de novo assemblies and metagenomics of viral populations. Moreover, SMRT sequencing can directly detect epigenetic modifications, making it useful in epigenetics studies.

About ProGenTomics

ProGenTomics, located in Gent (Belgium), offers a wide range of proteomics and genomics services and support to the academic community and its external customers. We aim to tailor our services to the needs of our collaborators and customers, while giving great support.  

Contact us

ProGenTomics, proteomics & genomics services

  Ghent University, Faculty of Pharmaceutical Sciences

  Ottergemsesteenweg 460, 9000 Gent, Belgium

 

  +32 9 264 83 56